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. 2022 Jan 18;13(2):2889–2901. doi: 10.1080/21655979.2022.2025700

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — HMGA1 overexpression relieved the effect of sh-TMPO-AS1 on CHOL cells. (a-b) qRT-PCR (a) and Western blotting (b) identified the expression of HMGA1 in HuCCT1 and RBE cells. (c) CCK8 assay detected cell proliferation in HuCCT1 and RBE cells. (d) Colony formation assay assessed the colony formation capability of HuCCT1 and RBE cells. (e) Flow cytometry assay measured the cell apoptosis rate of HuCCT1 and RBE cells. (f) Wound healing assay identified the change of cell migration in HuCCT1 and RBE cells. sh-TMPO-AS1, siRNA targeting TMPO-AS1. NC, negative control. pcDNA3.1-HMGA1, HMGA1 overexpression vectors. **P < 0.01 vs. sh-NC. #P < 0.05, ##P < 0.01 vs. sh-TMPO-AS1.