Fig. 5.

Production and characterization of the four nanobody‑vHRP fusions against S. Enteriditidis. a Detection of the four nanobody‑vHRP fusions expressed in the HEK293T cells by IFA. b Western blot analysis with anti-His monoclonal antibody. c The four nanobody‑vHRP fusions specifically binding with S. Enteriditidis by direct ELISA. d Determination the capacity of four nanobody‑vHRP fusions binding with the S. Enteriditidis by direct ELISA. e Identification the four nanobody‑vHRP fusions reaction with another strain of S. Enteriditidis, three Salmonella strains and five other non-Salmonella strains