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. 2022 Mar 31;20:167. doi: 10.1186/s12951-022-01376-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Development of the double nanobody‑based sandwich ELISA to detect S. Enteriditidis. a The Nb-pair comprising SE-Nb9 and SE-Nb1-vHRP were chosen as capturing and detecting antibodies, respectively. b The concentration of 10 μg/mL SE-Nb1 and SE-Nb1-vHRP (1:50) were optimized for detecting S. Enteriditidis. c The specificity and cross-reactivity analysis with two strains of S. Enteriditidis, three strains of other Salmonella and five other non-Salmonella strains. d The standard curve of the developed immunoassay to detect S. Enteriditidis