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. 2022 Mar 15;49(5):66. doi: 10.3892/ijmm.2022.5122

Figure 6.

Figure 6

Effect of histone inhibitor JIB04 on Cyld+/+ and Cyld−/− melanoma cells. Representative (A) western blot of H3K9me2 levels (relative to H3) following 24 h treatment with JIB04 (500 nM) and (B) anchorage-dependent clonogenic assay in murine cell lines. Representative (C) western blot of H3K9me2 levels (relative to H3) following 24 h treatment with JIB04 (500 nM) and (D) anchorage-dependent clonogenic assays of h cell lines (Cyld+/+:Cyld−/− colony size). JIB04, Jumonji histone demethylase inhibitor; Ctrl, control; h, human; m, mouse; CYLD, CYLD lysine 63 deubiquitinase; H3, histone 3.