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. 2022 Mar 15;49(5):66. doi: 10.3892/ijmm.2022.5122

Figure 7.

Figure 7

Effect of histone inhibitor CM272 on Cyld+/+ and Cyld−/− melanoma cells. Representative western blot of H3K9me2 levels following 24 h treatment with CM272 relative to H3 of (A) murine cell lines. Representative images of anchorage-dependent clonogenic assays of (B and C) murine cell lines (mCyld+/+:mCyld−/− colony size). Representative western blot of H3K9me2 levels following 24 h treatment with CM272 relative to H3 of (D) human cell lines and representative images of anchorage-dependent clonogenic assays of (E and F) human cell lines (hCYLD+:hCYLD− colony size). Representative western blot of CYLD protein expression following 24 h treatment with CM272 relative to β-actin of (G) murine and (H) human cells. (I) Analysis of the chromatin accessibility of the mCyld−/− cell lines after 24 h treatment with CM272 (200 nM) (quantification of relative chromatin accessibility to untreated mCyld−/− cells). *P<0.05. Ctrl, control; h, human; m, mouse; CYLD, CYLD lysine 63 deubiquitinase; H3, histone 3.