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. 2022 Feb 27;13(3):6464–6475. doi: 10.1080/21655979.2022.2031385

Figure 4.

Figure 4.

CircEHD2 competitively combines with miR-3186-3p which targets FOXK1.

(a). Bioinformatics website http://starbase.sysu.edu.cn/ to query circEHD2 and miR-3186-3p’s latent binding sites; B. RT-qPCR to examine miR-3186-3p expression in H520 cells transfected with si/oe-circEHD2; C. The luciferase activity assay verification of the targeting link of circEHD2 with miR-3186-3p; D. RT-qPCR to examine FOXK1 mRNA expression in NSCLC tissue and normal adjacent tissue; E. Western blot analysis of FOXK1 protein expression in BEAS-2B and NSCLC cell lines; F. Bioinformatics website http://starbase.sysu.edu.cn/ to query miR-3186-3p and FOXK1ʹs targeting link; G. Western blot detection of FOXK1 expression in H520 cells transfected with miR-3186-3p mimic; H. The luciferase activity assay verification of the targeting link of miR-3186-3p with FOXK1; Manifestation of the data was as mean ± SD (n = 3); *P < 0.05.