Figure 5.

CircEHD2 impacts NSCLC via controlling miR-3186-3p/FOXK1 pathway.

(a). Western blot to detect FOXK1 protein expression; B. Colony formation assay to detect cell proliferation; C. Edu assay to detect DNA replication; D. The apoptosis rate determined via flow cytometry; E, F. Glucose consumption and lactic acid production; G, H. RT-qPCR to detect HK-2 and GLS1 mRNA expression; I. LCB3, Beclin-1 and p62 protein expression detected via Western blot. In H520 cells co-transfected with si-circEHD2 with miR-3186-3p inhibitor or /oe-circEHD2 and si-FOXK1. Manifestation of the data was as mean ± SD (n = 3); *P < 0.05.