Figure 4.

LncRNA SNHG8 binds to miR-656-3p

(A) The binding sequences of lncRNA SNHG8 and miR-656-3p, as predicted by the bioinformatic databases.(B) RT-qPCR was performed to confirm the transfection efficiency of miR-656-3p mimics in A375 and A875 cells.(C) Luciferase reporter assay verified the binding of miR-656-3p to SERBP1 in A375 and A875 cells.(D and E) RT-qPCR was used to measure miR-656-3p expression in melanoma tissues and cells.(F) Pearson’s correlation analysis analyzed the negative relation between miR-656-3p and lncRNA SNHG8 in our cohort.(G) RT-qPCR showed miR-656-3p expression in melanoma cells transfected with sh-NC or sh-SNHG8.*P < 0.05; **P < 0.01