TABLE 1.
Properties of bacterial strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| E. coli strains | ||
| HB101 | supE44 hsdS20 (rB− mB−) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1 | Takara Shuzo |
| JM109 | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 Δ(lac-proAB)/F′ (traD36 proAB+ lacIqZΔM15) | Takara Shuzo |
| M15 | lac ara gal mtl F−recA+ uvr+ | Qiagen |
| Plasmids | ||
| pFBG1204 | pUC118ΩBamHI-BglII 2.9 kb; Ampr | This study (Fig. 1) |
| pFBG2204 | pUC119ΩBamHI-BglII 2.9 kb; Ampr | This study (Fig. 1) |
| pFBG1216 | Deleted plasmid derived from pFBG1204; Ampr | This study (Fig. 1) |
| pFBG1221 | Deleted plasmid derived from pFBG1204; Ampr | This study (Fig. 1) |
| pUC118 | Vector; Ampr | Takara Shuzo |
| pUC119 | Vector; Ampr | Takara Shuzo |
| pREP4 | neo lac1; Kmr | Qiagen |
| pQE30 | Vector; Ampr | Qiagen |
| pQEFA | pQE30ΩfomA; Ampr | This study (see text) |
| pQEFB | pQE30ΩfomB; Ampr | This study (see text) |
a
Ampr, ampicillin resistant; Kmr, kanamycin resistant.