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Kinetic properties of the fomA and fomB gene products
| Parameter | Value for:
|
|
|---|---|---|
| FomA | FomB | |
| Molecular mass (kDa) as determined by: | ||
| Deduced amino acid sequence | 29 | 37 |
| SDS-PAGE | 29 | 50 |
| Gel filtration | 110 | 75 |
| Km (μM) for: | ||
| Fosfomycin | 150 | |
| Fosfomycin monophosphate | 680 | |
| ATP | 120 | 560 |
| Vmax (μmol/s) | 0.050 | 0.12 |
| Optimum temp (°C) | 45 | 37 |
| Optimum pH | 7.2 | 7.5 |
