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. 2022 Jan 16;13(2):2308–2320. doi: 10.1080/21655979.2021.2024396

Figure 5.

Figure 5.

miR-515-5p targets SLIT3 directly in COAD. A. The wild-type putative binding sequences of miR-515-5p in the sequence of the SLIT3 3ʹ-UTR. B. SLIT3 3ʹ-UTR (WT and MUT) luciferase vectors were co-transfected with miR-515-5p mimic or mimic NC, the activity of SLIT3 3ʹ-UTR was measured by luciferase. Renilla was used as control. **P < 0.001 vs. mimic-NC. C. Detection of SLIT3 expression in SW480 and SW620 cells by RT-qPCR, **P < 0.001 vs. FHC. D. Detection of SLIT3 expression in COAD tissues by RT-qPCR. **P < 0.001. E. The relationship between SLIT3 mRNA and miR-515-5p in COAD tissues was determined by Pearson’s correlation analysis. F. Western blotting analysis of SLIT3 expression in SW480 and SW620 cells transfected with UBXN10-AS1 overexpression or silence. Empty vector, empty lentivirus vectors. UBXN10-AS1-OE, UBXN10-AS1-overexpressing lentivirus vectors. **P < 0.001 vs. empty vector, &&P < 0.001 vs. si-NC.