Figure 5.

FOXD3-AS1 promoted TMZ resistance through upregulation of WEE1 in U87 cells.

(A) Overexpression of WEE1 increased IC₅₀ of TMZ treatment in U87 cells. N = 3. (B) The colony formation assay showed that U87 cells with overexpression of WEE1 were more tolerant to TMZ treatment. Representative plates are shown. N = 3. (C) Depletion of WEE1 decreased the IC₅₀ of TMZ treatment in U87-R cells. N = 3. (D) The colony formation assay also indicated that U87-R cells with depleted WEE1 were more sensitive to TMZ treatment. Representative plates are shown. N = 3. (E) Depletion of WEE1 could reverse the TMZ tolerance of U87 cells induced by overexpression of FOXD3-AS1. After transfection with the indicated reagents, and exposure to 0, 10, 25, 50, 100, 200, 400, and 800 μM TMZ for six days, the survival rate of U87 cells was assessed by the MTT assay. N = 3. (F) Colony formation assay. U87 cells were subjected to specific reagent transfection and simultaneous TMZ exposure at 0 and 50 μM. Representative plates are shown. N = 3.