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. 2022 Feb 13;13(3):4885–4897. doi: 10.1080/21655979.2022.2036898

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — miR-449a engaged in the influences of silencing circCDK6 in HeLa and CaSki cells. The expression level of miR-449a (a) and circRNA CDK6 (b) in HeLa and CaSki cells transfected with si-circCDK6 and miR-449a inhibitor detected by qRT-PCR, the sample size was 1 μg/well. Influence of circCDK6 and miR-449a on cell viability assayed by CCK-8, the sample size was 1 × 10³/well(c), cell proliferation examined by colony formation assay, the sample size was 1 × 10³/well (d), migration (e) and invasion (f) detected by transwell assay(the sample size was 5 × 10⁴/well), and cell cycle detected by Flow cytometry(the sample size was 1 × 10⁶/well). *p < 0.05, **p < 0.01 and ***p < 0.001 vs. respective control.