Table 1.
CircRNA CDK6 | FORWARD | CCAAGTTTTAGCAGAAAACCTCTCC |
REVERSE | CTCCTCGAAGCGAAGTCCTC | |
GAPDH | FORWARD | ATGGGGAAGGTGAAGGTCG |
REVERSE | TTACTCCTTGGAGGCCATGTG | |
linear mRNA | FORWARD | CAGTGACTTTCCTCTGACATGC |
REVERSE | GGCTCTGCATAAAACGGCAC | |
GAPDH | FORWARD | ATGGGGAAGGTGAAGGTCG |
REVERSE | TTACTCCTTGGAGGCCATGTG | |
si-CircRNA CDK6-Homo1 | Sense: | 5’- AAGUUUUAGCAGAAAACCUCUTT |
Antisense: | 5’-AGAGGUUUUCUGCUAAAACUUTT | |
si-CircRNA CDK6-Homo1 | Sense: | 5’- CCAAGUUUUAGCAGAAAACCUTT |
Antisense: | 5’-AGGUUUUCUGCUAAAACUUGGTT | |
si-CircRNA CDK6-Homo1 | Sense: | 5’- CAGAAAACCUCUCCGCGCGAATT |
Antisense: | 5’- UUCGCGCGGAGAGGUUUUCUGTT | |
Negtive control | Sense: | 5’-AUGUUUAAAUUGUUGUGAAATT |
Antisense: | 5’-UUUCACAACAAUUUAAACAUTT | |
miR-449a | FORWARD | CGCGTGGCAGTGTATTGTTA |
miR-449a | REVERSE | AGTGCAGGGTCCGAGGTATT |
miR-449a | RT Primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCAGC |
U6 | FORWARD | CTCGCTTCGGCAGCACATATACT |
U6 | REVERSE | ACGCTTCACGAATTTGCGTGTC |
U6 | RT Primer | AAAATATGGAACGCTTCACGAATTTG |