Figure 3.

Knockdown of ZFAS1 inhibits osteosarcoma cells proliferation.

(a) Knockdown of ZFAS1 reduced U2OS cells viability. n = 3, *p < 0.05. (b) Knockdown of ZFAS1 reduced MG63 cells viability. n = 3, *p < 0.05. (c) Knockdown of ZFAS1 inhibited colony formation of U2OS cells. Representative plates were shown. n = 3, *p < 0.05. (d) Knockdown of ZFAS1 inhibited colony formation of MG63 cells. Representative plates were shown. n = 3, *p < 0.05. (e) U2OS cells transfected with ZFAS1 siRNA or control siRNA were immunofluorescence stained with Ki-67. Representative images were shown. n = 3. (f) MG63 cells transfected with ZFAS1 siRNA or control siRNA were immunofluorescence stained with Ki-67. Representative images were shown. n = 3. (g) Levels of PCNA, CDK1, CDK4 CDK6, p53, and p16 in U2OS cells or MG63 cells transfected with ZFAS1 siRNA or control siRNA were examined by immunoblotting. Actin was served as the loading control. Representative blots were shown. n = 3.