Figure 3.

The effects of miR-15b-5p on TNF-α-induced inflammatory response and ECM deposition in ASM cells.

ASM cells were transiently transfected with miR-15b-5p mimic or mimics-NC, and treated with 10 ng/ml of TNF-α for 24 h. (a) The levels of IL-6 and IL-8 in ASM cells were measured by ELISA. (b)The expression of collagen I and collagen III proteins in ASM cells were detected by Western blot assay. Data are displayed as mean ± standard deviation (n = 3 independent experiments). ***P < 0.001 versus blank group or mimics-NC + TNF-α group.