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. 2022 Jan 26;13(2):3410–3421. doi: 10.1080/21655979.2022.2027062

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Effects of circ_0033596 overexpression or knockdown on HUVEC viability, cell cycle progression and apoptosis.

(a) Circ_0033596 overexpression plasmid or si-circ_0033596#1/2 was transfected into ox-LDL-induced HUVECs, and qRT-PCR was employed to detect circ_0033596 expression to verify the transfection efficiency. (b) HUVEC viability was detected by CCK-8 assay after the transfection. (c) The cell cycle progression of HUVECs was detected via flow cytometry after the transfection. (d) Flow cytometry was utilized to detect HUVEC apoptosis after the transfection.*P < 0.05, **P < 0.01, and ***P < 0.001.