Figure 4.

Circ_0033596 modulates the viability, cell cycle progression and apoptosis of HUVECs via targeting miR-217-5p.

(a) Ox-LDL-induced HUVECs were transfected with circ_0033596 overexpression plasmids and miR-217-5p mimics, and qRT-PCR was utilized to detect miR-217-5p expression to verify the transfection efficiency. (b) Circ_0033596 overexpression plasmids and miR-217-5p mimics were transfected into ox-LDL-induced HUVECs, and the cell viability was detected using CCK-8 assay. (c) The cell cycle progression of HUVECs was detected through flow cytometry after the transfection. (d) Flow cytometry was employed to detect the apoptosis of HUVECs after the transfection.*P < 0.05, **P < 0.01 and ***P < 0.001.