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. 2022 Feb 15;13(3):5251–5261. doi: 10.1080/21655979.2022.2037870

Figure 1.

Figure 1.

circRNA_0000285 serves as a sponge for miR-654-3p. (a) Predicted circRNA_0000285 binding site within the miR-654-3p WT or MUT 3ʹ-UTR. (b) Relative luciferase activity detected following the co-transfection of the luciferase reporter construct containing the miR-654-3p 3ʹ-UTR sequence with the WT or MUT circRNA_0000285 binding site. (c-e) circRNA_0000285-specific probe used to pull-down circRNA_0000285 and enrich miR-654-3p. P < 0.05, **P < 0.01 vs. control.