Figure 1.
circRNA_0000285 serves as a sponge for miR-654-3p. (a) Predicted circRNA_0000285 binding site within the miR-654-3p WT or MUT 3ʹ-UTR. (b) Relative luciferase activity detected following the co-transfection of the luciferase reporter construct containing the miR-654-3p 3ʹ-UTR sequence with the WT or MUT circRNA_0000285 binding site. (c-e) circRNA_0000285-specific probe used to pull-down circRNA_0000285 and enrich miR-654-3p. P < 0.05, **P < 0.01 vs. control.