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. 2022 Feb 15;13(3):5251–5261. doi: 10.1080/21655979.2022.2037870

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Knockdown of circRNA_0000258 regulates SiHa cell viability and apoptosis via upregulation of miR-654-3p expression. SiHa cells were transfected with control-siRNA, circ-siRNA, circ-siRNA + inhibitor control or circ-siRNA + miR-654-3p inhibitor. (a) Effect of circ-siRNA transfection for 48 h on SiHa cell viability evaluated by MTT assay. (b, c) Effect of circ-siRNA on SiHa cell apoptosis analyzed using flow cytometry. (d) Western blotting to detect Bax and Bcl-2 protein expression. (e) Ratio of Bax/Bcl-2. P < 0.05, **P < 0.01 vs. control.