Figure 5.

E3 ubiquitin ligase Smurf1 affects PTEN expression through ubiquitination. A: WB detected the PTEN protein level in myocardial tissues of CHF rats; B: IP experiment found that Smurf1 could be combined with PTEN; C: IP test was used to detect the changes of PTEN ubiquitination after Smurf1 silencing; D: The changes of PTEN ubiquitination after Smurf1 overexpression were detected by IP; E: Expressions of Smurf1 and PTEN in cells detected by WB; N = 10; Values in the figure were measurement data and displayed as mean ± standard deviation. Unpaired t test was used for pairwise comparisons; one-way ANOVA was used for comparisons among multiple groups, followed by Tukey’s test. * P < 0.05.