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Primer sequences for qRT-PCR
| Gene | Primer sequences |
|---|---|
| miR-129-5p | F: 5ʹ-GATCCGCAAGCCCAGACCGCAAAAAGTTTTTA-3’ |
| R: 5ʹ-AGCTTAAAAACTTTTTGCGGTCTGGGCTTGCG-3’ | |
| Smurf1 | F: 5ʹ-AGTTCGTGGCCAAATAGTGG-3’ |
| R: 5ʹ-GTTCCTTCGTTCTCCAGCAG-3’ | |
| PTEN | F: 5ʹ-CAGATTATGGAATGTAGGCGGCTTGA-3’ |
| R: 5ʹ-TGGCAATAGCCGAACAGTTCT-3’ | |
| GAPDH | F: 5ʹ-AGTGCCAGCCTCGTCTCATA-3’ |
| R: 5ʹ-GGTAACCAGGCGTCCGATAC-3’ | |
| U6 | F: 5ʹ-CTCGCTTCGGCAGCACA-3’ |
| R: 5ʹ-AACGCTTCACGAATTTGCGT-3’ |
