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. 2022 Feb 7;13(2):2992–3006. doi: 10.1080/21655979.2021.2017679

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Polygonum cuspidatum inhibited OS cells growth and suppressed its viability. (a) & (b) The CCK-8 assay was carried out to evaluate OS cell viability upon polygonum cuspidatum treatments with different dosages (0,3.125,6.25,12.5,25,50,100 μg/mL) in 24 h and 48 h. (c) & (d) CCK-8 assay was conducted to assess OS cell growth ability after treating with polygonum cuspidatum at different dosages (8 or 13 μg/mL) in 24 h, 48 h and 72 h. (e–h) Wound-healing assay was used to evaluate the cell motility after polygonum cuspidatum treatment for 24 h. *p < 0.05 by Student’s t-test. (i–l) Cell colony formation assay was carried out to access the cell clonality after polygonum cuspidatum treatment for 24 h. **p < 0.01 was based on the Student’s t-test.