Figure 2.

MiR-195-5p was the target of LINC00662.

a. qRT-PCR was used to detect LINC00662 expression in nuclear and cytoplasmic fractions of BGC823 and SGC-7901 cells. B. The binding sequences between miR-195-5p and LINC00662 were predicted. c. Luciferase activities of the cells were detected to validate the predicted binding sites. d-e. RNA pull-down assay was adopted to validate the interaction between miR-195-5p and LINC00662. F. qRT-PCR data were utilized to examine the correlation between miR-195-5p expression and LINC00662 expression in GC samples (N = 45). g. LINC00662 overexpression plasmids were transfected into BGC823 cells, and siRNAs were transfected into SGC-7901 cells, and then the expression of LINC00662 in GC cells was detected by qRT-PCR. h. qRT-PCR was utilized to quantify miR-195-5p expression in GC cell lines after transfection. All of the experiments were performed in triplicate. **P < 0.01, ***P < 0.001 and ns: P > 0.05.