Figure 4.

CEP55 was the direct target of miR-195-5p.

a. Bioinformatics analysis was employed for predicting the binding sequences between miR-195-5p and 3ʹUTR of CEP55. B. Luciferase activity was performed to validate the predicted binding sites after reporter vectors and miR-195-5p mimics (or miR-con) were co-transfected into the cells. c. qRT-PCR was adopted to quantify miR-195-5p in GC cells after the cells were transfected with miR-195-5p mimics or inhibitors. d-e. CEP55 mRNA and protein in GC cells with miR-195-5p overexpressed or inhibited were quantified by qRT-PCR (d) and Western blot (e). F. qRT-PCR was utilized to detect the expression level of CEP55 mRNA in GC tissues (N = 45) and non-cancerous tissues (N = 45). g. Western blot was adopted to quantify CEP55 protein in GC tissues (N = 3) and non-cancerous tissues (N = 3). h. The association between miR-195-5p and CEP55 mRNA expressions in GC samples (N = 45) was evaluated. All of the experiments were performed in triplicate. ***P < 0.01 and ns: P > 0.05.