Figure 5.

LINC00662 participated in regulating biological behaviors of GC cells by modulating CEP55 expression.

a. The correlation between LINC00662 and CEP55 mRNA expressions in GC samples (N = 45) was evaluated. b-d. BGC823 cells were transfected with LINC00662 overexpression plasmid or co-transfected with si-CEP55, respectively, and SGC-7901 cells were transfected with si-LINC00662#3 or co-transfected with CEP55 overexpression plasmid. CEP55 mRNA and protein were quantified by qRT-PCR (c) and Western blot (d). e-f. The proliferation of transfected cells was measured employing CCK-8 assay (E) and BrdU assay (F).G. Transwell experiment was conducted to measure the migration and invasion of transfected cells. All of the experiments were performed in triplicate. *P < 0.05, **P < 0.01 and ***P < 0.001.