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. 2022 Feb 13;13(2):4271–4284. doi: 10.1080/21655979.2022.2031389

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — miR-148b-3p directly bind to SOS1. (a) The possible target site of miR-148b-3p on SOS1-wt were predicted by Targetscan online. (b) Relative luciferase activities in MG-63 cells was examined employing dual luciferase reporter gene. (c) Relative luciferase activities in U2OS cells was examined employing dual luciferase reporter gene. (d) The expression level of SOS1 in MG-63 cells was analyzed using qRT-PCR. (e) The expression level of SOS1 in U2OS cells was analyzed using qRT-PCR. (f) The expression of SOS1 in MG-63 and U2OS was analyzed by WB assay. **P < 0.01 versus miR-NC; ^##P < 0.01 versus miR-148b-3p.