Figure 1.

SPAG5 expression was increased in MM tissues and samples. (a) SPAG5 expression in MM tissues and normal tissues, as predicted in GEPIA database. (b) SPAG5 mRNA levels in 60 paired MM tissues and normal tissues were determined using qRT-PCR. (c, d) The protein levels of SPAG5 in 3 paired MM tissues and normal tissues were determined using Western blotting and IHC analysis. (e, f) The mRNA and protein expression of SPAG5 in NHDF, A375, A2058, WM115 and MV3 cell were measured by qRT-PCR and Western blotting. (n = 3, *P < 0.05, **P < 0.01).