Figure 2.

Deletion of SPAG5 inhibited MM cell growth and induced a G1 phase arrest. A375 and A2058 cells were transfected with shNC, shSPAG5-1 and shSPAG5-2, and submitted to the following assays. (a) SPAG5 expression was detected by using Western blotting assay. (b) CCK-8 was used for cell proliferation detection. (c) Colony formation assay was performed for cell viability test. (d) EdU staining was used for cell proliferation test. (e) Cell cycle distribution was assessed using flow cytometry assay. (n = 3, *P < 0.05, **P < 0.01).