Table 6.

Gelatin culture scaffold

Crosslinkers	Mechanical properties	Porosity	Pore size	Type of cultured cells	Medium used	Maximum growth rate	References
Into hydrogel form	N/A	2.99%	4 to 6 µm	HeLa cells (immortalized human cervical cancer cells)	Dulbecco’s modified eagle’s medium (DMEM)	Cell viability of 95%	[51]
N/A	1.26 ± 0.16 MPa	close to 90%	102.7 ± 39.9 µm	Mesothelial Cells and Mesothelium Tissue	Dulbecco’s Modified Eagles Medium (DMEM) and fetal bovine serum (FBS)	DNA content of cells: 1.38 µg	[52]
Ethylene dichloride (EDC)/N-hydroxysuccinimide (NHS)	N/A	N/A	N/A	Cornea epithelial cells	Dulbecco’s Modified Eagles Medium	Cell number: 2.8 × 104 to 3.8 × 104 cells	[53]
EDC/NHS	4 MPa	N/A	N/A	Endothelial cells and smooth muscle cells	Fetal calf serum (FCS) and Minimum Essential Medium	Increased significantly in HE staining results	[54]