Figure 4.

4-PBA alleviated RFP-caused increase of AP2 and clathrin.

(a and b) HepG2 cells were treated by RFP (50 uM) and/or 4-PBA (1 mM) for 48 h. The mRNA and protein expressions of AP2 and clathrin in cells were tested via qRT-PCR and Western blotting. (c and d) Following CHOP-siRNA (or CHOP-plasmid) transfection and/or RFP (50 uM) or 4-PBA (1 mM) treatment for 48 h, the CHOP mRNA expression was tested via qRT-PCR assay and the protein expressions of AP2 and clathrin in HepG2 cells were tested via Western blotting. *P< 0.05, **P< 0.01, ***P< 0.001 vs. control group; ^#P< 0.05, ^##P< 0.01, ^###P< 0.001 vs. RFP group; ^&P< 0.05, ^&&&P< 0.001 vs. 4-PBA group.