Figure 2.

TET reduced oxidative stress and apoptosis in TBHP-treated NP cells. After treatment with TET (0, 0.1, 0.5, 1, 5, 10 μM) for 2 h, NP cells were administered for 24 h with TBHP (75 μM), and the same amount of normal saline was used as control. (a-c) ROS, MDA and SOD contents were evaluated by ELISA. (d) NP cells apoptosis was measured by flow cytometry. (e) Apoptosis rate was showed in a histogram (f) Cleaved caspase 3 and cleaved caspase 9 expression were tested by Western blot. (g-h) THE relative expression of cleaved caspase 3 and cleaved caspase 9 were showed in histograms. **p < 0.01 vs. control group, #p < 0.05 vs. TBHP group, ##p < 0.01 vs. TBHP group.