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. 2022 Feb 2;13(2):3944–3957. doi: 10.1080/21655979.2022.2031396

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — TET delayed rat intervertebral disc degeneration induced by puncture in annulus fibrosus. (a-b) The pathological changes of intervertebral disc were detected by HE and Safranin-O staining. (c-f) LC3 and Collagen II expression in intervertebral disc were measured by immunohistochemistry, and the relative expression were showed in histograms. (g) The apoptosis was assessed by TUNEL assay. (h) Relative apoptosis level was showed in a histogram. **p < 0.01 vs. control group, ##p < 0.01 vs. TBHP group.