Downregulation of miR-3098-3p reverses the effects of circ-Carm1 deficiency on cell viability and ferroptosis of HT22 cells. (a) miR-3098-3p expression levels were detected using RT-qPCR after transfection. (b) Cell viability of HT22 cells co-transfected with si-circ-Carm1 and miR-3098-3p inhibitor and was detected using the CCK-8 assay. (c-d) TUNEL staining was conducted to detect cell death in cells co-transfected with si-circ-Carm1 and miR-3098-3p inhibitor. (e-f) Levels of Fe2+ and malondialdehyde (MDA) were determined using an enzyme-linked immunosorbent assay. (g-j) Protein levels of transferrin receptor 1 (TFR1), glutathione peroxidase 4 (GPX4), and glutathione (GSH) were detected by Western blotting.
**P < 0.01, ***P < 0.001,vs. NC mimic and control group. #P < 0.05, ##P < 0.01, ###P < 0.001, vs. NC inhibitor and OGD/R group. &P < 0.05, &&P < 0.01, vs. OGD/R + si-circ-Carm1 + NC inhibitor group.