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. 2022 Feb 3;13(2):4411–4427. doi: 10.1080/21655979.2022.2033447

Figure 3.

Figure 3.

MiR-637 is the circ_UBAP2 target.a) qRT-PCR detected subcellular localization of circ_UBAP2 in HOS cells. b) qRT-PCR detected subcellular localization of circ_UBAP2 in SaOS-2 cells. c) Binding sequences of miR-637 with circ_UBAP2 predicted with bioinformatics. d) Targeting relation of miR-637 with circ_UBAP2 was measured through dual-luciferase reporter assay within HOS cells. e) Targeting relation of miR-637 with circ_UBAP2 was measured through dual-luciferase reporter assay within SaOS-2 cells. f) qRT-PCR detected expression of streptavidin-captured circ_UBAP2 after biotin-labeled miR-637 (bio-wt-miR-637), bio-mut-miR-637 or negative control (bio-NC) were transfected into OS cells. g) miR-637 levels within OS cells were detected through qRT-PCR upon circ_UBAP2 over-expression within HOS cells or silencing within SaOS-2 cells. * P < 0.05, ** P < 0.01.