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. 2022 Jan 22;13(2):3503–3515. doi: 10.1080/21655979.2022.2029108

Figure 3.

Figure 3.

MiR-21-5p directly targeted SMAD7 in keloid fibroblasts. (a) SMAD7 contained a conserved binding site of miR-21-5p. (b) The binding of SMAD7 and miR-21-5p was verified by dual-luciferase reporter gene assay. (c) qRT-PCR was applied to detect the expression of SMAD7 in keloid tissue. (d) qRT-PCR was applied to detect the expression of SMAD7 in keloid fibroblasts. (e) Pearson correlation analysis was used to detect the correlation between SMAD7 and miR-21-5p in keloid tissue. (f) qRT-PCR was used to detect the level of SMAD7 after overexpression of miR-21-5p. Cotransfection of miR-21-5p inhibitors and si-SMAD7 into keloid fibroblasts. (g) The levels of miR-21-5p and SMAD7 were detected by qRT-PCR. (h) MTT assay was applied to assess the cell viability of keloid fibroblasts. (i) Flow cytometry was used to detect cell apoptosis of keloid fibroblasts. (j) Transwell assay was applied to assess the cell migration and invasion of keloid fibroblasts. * P < 0.05, ** P < 0.01, *** P < 0.001.