Figure 4.

RPN2 functioned via modulating ROS level in LSCC cells. (a) Intracellular ROS levels were examined by DCFH-DA fluorescence. (b) AMC-HN-8 cells transfected with RPN2 overexpressing plasmid were treated with 10 mmol/l NAC for 24 h. Cell migration was assayed by Transwell analysis. (c) Protein levels of E-cadherin and Vimentin were assayed by Western blot in AMC-HN-8 cells after different treatments. (d) Glucose uptake was measured in AMC-HN-8 cells. (e) Lactate production release was evaluated in AMC-HN-8 cells. *P < 0.05.