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. 2022 Jan 16;13(2):2017–2027. doi: 10.1080/21655979.2021.2012552

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Overexpression of SIRT3 attenuated USP interfere-induced cell senescence.

SIRT3 was overexpressed in USP3-silencing rat primary chondrocytes. (A–B) Confirmation of SIRT3 overexpression using qPCR (A) and Western blotting (B) ***p < 0.001 vs. vector. (C) Cell cycle detection by flow cytometry. (D) ROS levels measured by flow cytometry. (E) Cell senescence shown by SA-β-Ga1 staining. (F) Expressions of SIRT3, p16, p21, Cyclin E1, CDK2, FOXO3, Ac-FOXO3, catalase, and Mn-SOD detected using Western blotting. Each data shows mean ± standard deviation from three independent biological experiments. *p < 0.05; **p < 0.01; ***p < 0.001 compared with siNC; #p < 0.05; ##p < 0.01; ###p < 0.001 compared with siUSP3-1 + vector.