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. 2022 Mar 7;13(3):6942–6954. doi: 10.1080/21655979.2022.2036398

Figure 2.

Figure 2.

Overexpression of miR-193b-3p inhibits oxidative stress and mitochondrial apoptosis of neurons in the cerebral cortex of rats. The miR-193b-3p agomir was injected into the lateral ventricle of rats to induce the overexpression of miR-193b-3p, and the rat brain I/R injury model was established by suture method. After 2 h of ischemia and 24 h of reperfusion, the rat brain tissues were collected for relevant detection. (A) DCFH-DA fluorescent probe was used to detect ROS levels in neurons of rat brain tissue; (B) SOD level of neurons in rat brain tissue was detected by the kit; (C) MDA level of neurons in rat brain tissue was detected by the kit; (D) JC-1 fluorescence probe was used to detect MMP of neuron mitochondria in rat brain; (E) The level of mitochondrial ATP in rat brain tissue was detected by kits. Measurement data were expressed as mean ± standard deviation, N = 6, one-way ANOVA was used for data comparison among groups, and Tukey’s multiple test was used for the post hoc test. P was obtained from a bilateral test, ** P < 0.01, *** P < 0.001.