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. 2022 Mar 7;13(3):6942–6954. doi: 10.1080/21655979.2022.2036398

Figure 4.

Figure 4.

Overexpression of SIAH1 antagonizes the protective effect of overexpression of miR-193b-3p on cerebral I/R injury in rats. The SIAH1 was overexpressed in rats by injecting the SIAH1 lentivirus. (A) The level of SIAH1 was detected by RT-qPCR and WB. (B) DCFH-DA fluorescent probe was used to detect ROS levels in rat brain tissue; (C) SOD level of neurons in rat brain tissue was detected by the kit; (D) MDA level of neurons in rat brain tissue was detected by the kit; (E) JC-1 fluorescence probe was used to detect MMP of neuron mitochondria in rat brain tissue. (F) The kit was used to detect the level of mitochondrial ATP of neurons in rat brain tissue; (G) HE staining and TTC staining were used to detect the neuronal damage and cerebral infarct volume of rat brain tissue; (H) TUNEL staining was used to detect neuronal apoptosis in rats. Measurement data were expressed as mean ± standard deviation, N = 6, one-way ANOVA was used for data comparison among groups, and Tukey’s multiple test was used for the post hoc test. P was obtained from a bilateral test, ** P < 0.01, *** P < 0.001.