Table 4.
Median Expression of PDCD1, PDCD1LG1, PDCD1LG2 and Their Splicing Variants
| Splicing Variant | PCs | BMMCs | Statistical Significance (p) |
|---|---|---|---|
| fl_PD-1 | 0.0803 | 0.0989 | 0.0006 |
| Δex2_PD-1 | 0.0151 | 0.0703 | 0.0001 |
| Δex3_PD-1 | 0.0648 | 0.0787 | 0.0002 |
| Δex2,3_PD-1 | 0.000 | 0.0610 | 0.0013 |
| Δex2,3,4_PD-1 | 0.0629 | 0.0691 | 0.0185 |
| fl_PD-L1 | 0.2495 | 0.1507 | < 0.0001 |
| Δex2_PD-L1 | 0.2603 | 0.1437 | < 0.0001 |
| fl_PD-L2 | 0.1471 | 0.1011 | < 0.0001 |
| Δex3_PD-L2 | 0.1404 | 0.0817 | < 0.0001 |
| Δex3,fs_PD-L2 | 0.0669 | 0.0567 | 0.0126 |
Notes: The PDCD1, PDCD1LG1, PDCD1LG2 and their splicing variants mRNA expression was calculated as inverse ratio of the difference in cycle threshold (1/ΔCt) method. ΔCt is the difference between the Ct value of the target gene and the Ct value of GAPDH gene.
Abbreviations: PCs, plasma cells; BMMCs, bone marrow mononuclear cells.