TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid(s) | Relevant properties | Source (reference) |
|---|---|---|
| P. aeruginosa | ||
| PAO4009 | leu-9018 nir-9006; FP5+ | H. Matsumoto (7) |
| KH4014a | nfxC mutant of PAO4009 | H. Fukuda (3) |
| PAO4290 | leu-10 argF10 aph-9004; FP− | H. Matsumoto (28) |
| TNP070 | ΔmexA; derivative of PAO4290 | H. Yoneyama (28) |
| TNP071 | ΔmexB; derivative of PAO4290 | H. Yoneyama (28) |
| TNP072 | ΔoprM; derivative of PAO4290 | H. Yoneyama (28) |
| TNP073 | ΔmexA ΔmexB; derivative of PAO4290 | H. Yoneyama (28) |
| TNP077 | ΔmexA ΔmexB ΔoprM; derivative of PAO4290 | This study |
| TNP0701 | TNP070 derivative harboring pMEXA1 | H. Yoneyama (27) |
| TNP0705 | TNP070 derivative harboring pMEXE1 | This study |
| TNP0703 | TNP070 derivative harboring pMMB67HE | H. Yoneyama (27) |
| TNP0711 | TNP071 derivative harboring pMEXB1 | H. Yoneyama (27) |
| TNP0715 | TNP071 derivative harboring pMEXF1 | This study |
| TNP0713 | TNP071 derivative harboring pMMB67EH | H. Yoneyama (27) |
| TNP0721 | TNP072 derivative harboring pOPRM1 | H. Yoneyama (27) |
| TNP0725 | TNP072 derivative harboring pOPRN1 | This study |
| TNP0723 | TNP072 derivative harboring pMMB67EH | H. Yoneyama (27) |
| TNP0731 | TNP073 derivative harboring pMEXAB1 | This study |
| TNP0735 | TNP073 derivative harboring pMEXEF1 | This study |
| TNP0733 | TNP073 derivative harboring pMMB67EH | H. Yoneyama (27) |
| TNP0771 | TNP077 derivative harboring pMEXAB-OPRM1 | This study |
| TNP0775 | TNP077 derivative harboring pMEXEF-OPRN1 | This study |
| TNP0773 | TNP077 derivative harboring pMMB67EH | This study |
| TNP0776 | TNP077 derivative harboring pVLT33 | This study |
| TNP0777 | TNP077 derivative harboring pMEXEF-OPRN-KM1 | This study |
| E. coli | ||
| XL10 Gold | E. coli strain for transformation | Stratagene |
| S17-1 | Mobilizer strain | R. Simon (25) |
| Plasmids | ||
| pUC19 | Cloning vector; Ampr | Toyobo |
| pNOT19 | pUC19 derivative with NotI site; Ampr | H. P. Schweizer (24) |
| pKF18K | pUC18 derivative cloning vector; Ampr | Toyobo |
| pBluescript II SK(+) | Cloning vector; Ampr | Stratagene |
| pMOB3 | pHSS21 derivative carrying MOB cassette; Cmr Kmr | H. P. Schweizer (24) |
| pQE30, pQE31 | Expression vectors carrying a six-His affinity tag; Ampr | Qiagen, Inc. |
| pMMB67EH/HE | Broad-host-range vector; Ampr IncQ | Fürste (4) |
| pVLT33 | Broad-host-range vector; Kmr IncQ | V. de Lorenzo (2) |
| pMEXA1 | pMMB67EH derivative carrying mexA gene | H. Yoneyama (27) |
| pMEXB1 | pMMB67EH derivative carrying mexB gene | H. Yoneyama (27) |
| pOPRM1 | pMMB67EH derivative carrying oprM gene | H. Yoneyama (27) |
| pMEXE1 | pMMB67EH derivative carrying mexE gene | This study |
| pMEXF1 | pMMB67EH derivative carrying mexF gene | This study |
| pOPRN1 | pMMB67EH derivative carrying oprN gene | This study |
| pMEXAB1 | pMMB67EH derivative carrying mexA and mexB genes | This study |
| pMEXEF1 | pMMB67EH derivative carrying mexE and mexF genes | This study |
| pMEXAB-OPRM1 | pMMB67EH derivative carrying mexA, mexB, and oprM genes | This study |
| pMEXEF-OPRN1 | pMMB67EH derivative carrying mexE, mexF, and oprN genes | This study |
| pMEXEF-OPRN-KM1 | pVLT33 derivative carrying mexE, mexF, and oprN genes | This study |
| pΔMexA-B-OprM | pNOT19 derivative carrying defective mexA, mexB, and oprM; Ampr Kmr Cmr | This study |