FIG. 1.

Light microscopy appearance of paraffin sections of kidney cortex. (A and C) Sections stained with hematoxylin and eosin; (B and D) sections stained with methyl green-pyronine (Brachet staining [8]). Animals were treated with gentamicin for 10 days (10 [A and B] or 20 [C and D] mg/kg). Single arrows point to cells showing clear evidence of nuclear alterations related to apoptosis; the double arrowhead (in panel C) shows a cell with clear shrinkage and eosinophilia of the cytoplasm; NT (in panel C) denotes a necrotic tubule. Two mitotic figures are circled in panels B and D. Bars, 20 μm.