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. 2021 Dec 3;2:786034. doi: 10.3389/falgy.2021.786034

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Copyright © 2021 Bignold and Johnson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Type 2 inflammatory mediators stimulate pericyte migration. (A) Scratch assay performed on pericytes that were grown in pericyte medium and treated with 10 ng/ml of TGF-β, 100 ng/ml of IL-13 or 100 ng/ml of periostin for 48 h. Cells were transferred into serum-free medium and a scratch was made in each monolayer using a p200 pipette tip. (B) Images were taken at 100x magnification immediately after scratching and 24 h later. The scratch width was determined using ImageJ and the average distance of cell migration was calculated. n = 6 representative of two independent experiments, *p < 0.05 vs. untreated cells.