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. 2022 Feb 25;3:822554. doi: 10.3389/falgy.2022.822554

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Copyright © 2022 Gabler, Gebhard, Norwig, Eberlein, Biedermann, Brockow and Scherf.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of allergen test solutions. Gluten G and G-ω5 as well as sHWPs and eHWPs were analyzed. Protein marker (M) 3.5 μg, samples 5.3 μg (a), and 15.0 μg (b). HMW-GS, high-molecular-weight glutenin subunits, LMW-GS, low-molecular-weight glutenin subunits. The albumin/globulin fraction may consist of, e.g., grain softness proteins, puroindolines, purothionins (Tri a 37), non-specific lipid-transfer protein (Tri a 14), and amylase/trypsin-inhibitors (Tri a 15, Tri a 28, Tri a 29, Tri a 30, and Tri a 40) (40).