Figure 2.

Inhibition of TNFα-induced-chemokines by fluticasone in ASM cells pre-treated with conditioned media from 24-h activated mast cells. Healthy ASM cells were pre-treated with control mast cells media, 24-h non-activated and activated mast cell conditioned media. ASM cells were then washed and stimulated with TNFα (10 ng/ml) with or without fluticasone (100 nM) for 24 h. Expression of CCL5 (A), CXCL10 (B), and CXCL8 (C) was assessed by ELISA with data presented as % of the chemokine responses in cells treated with TNFα alone (Means ± SEM of n = 6 different cell lines) (*p < 0.05). Comparisons between groups were made using one-way ANOVA and Tukey Test correction.