Figure 3.

Inhibition of TNFα-induced chemokine mRNA expression by fluticasone in ASM cells pre-treated with conditioned media from 24-h activated mast cells. Healthy ASM cells were pre-treated with control mast cell media, conditioned media from 24-h non-activated, or 24-h activated mast cells for 24 h. ASM cells were then washed and stimulated with 10 ng/ml TNF-α with or without 100 nM fluticasone for 8 h. Chemokine expression of CCL5 (A), CXCL10 (B), and CXCL8 (C) was assessed by real-time PCR. Data are presented as % inhibition from chemokine responses in cells treated with TNFα alone (Means ± SEM of n = 5 different cell lines, *p < 0.05). Comparisons between groups were made using one-way ANOVA and Tukey Test correction.