Figure 4.

TGFβ impaired the capacity of fluticasone to inhibit TNFα-induced chemokine production. Healthy ASM cells were pre-treated with TGFβ (10 ng/ml) for 24 h before being stimulated with 10 ng/ml TNF-α with or without 100 nM fluticasone for 24 h. Chemokine expression of CCL5 (A) and CXCL10 (B) was assessed by ELISA with data presented as Means ± SEM of n = 3 different cell lines. Data are presented as % inhibition from chemokine responses in cells treated with TNFα alone or TNFα/TGFβ (Means ± SEM of n = 5 different cell lines, *p < 0.05). Comparisons between groups were made using paired student t-test.