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Competitive ELISA assay for GOS-specific IgE. Sera (n = 4) were pre-incubated with various doses of lactose, TDG, GOS, or allergenic GOS tetrasaccharides (GOS fraction) before the detection of GOS-specific IgE by ELISA. Graph (A–D) represent subjects S7, S8, S4, and S9, respectively.
