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. 2022 Feb 28;3:840454. doi: 10.3389/falgy.2022.840454

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Copyright © 2022 Lee, Leow, Wen, Soh, Chiang, Zhong, Tham, Loh, Delsing, Lee and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Basophil activation assay performed using purified basophils. Whole blood or purified basophils (n = 4) were stimulated with 100 μg/ml of GOS (left), 1 μg/ml of anti-IgE (middle), or 0.3 μg/ml of Blo t (right). The expression of CD63⁺ on basophils was analyzed by flow cytometry.